SubtiBank SubtiBank
opuE [2018-02-09T23:06:00.000Z]
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opuE [2018-02-09T23:06:00.000Z]

proline transporter
locus
BSU06660
pI
9.81
mw
53.12 kDa
protein length
492 aa Sequence Blast
gene length
1476 bp Sequence Blast
function
compatible solute transport
product
proline transporter
essential
no
synonyms
yerK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.1|Solute:sodium symporter family]
  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.7|Sodium-solute symporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.9|Uptake of compatible solutes]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    726,840 → 728,318

    Phenotypes of a mutant

  • strong growth disadvantage in high-salinity minimal media lacking proline [Pubmed|22685134]

    • The protein

    Protein family

  • solute:sodium symporter family (SSS family)

    • Paralogous protein(s)

  • [protein|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|PutP]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|9701821], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|22900538], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induced by glucose ([protein|search|CcpA]) [Pubmed|22900538]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
    • view in new tab

    Biological materials

    Mutant

  • available in [SW|Erhard Bremer]'s lab [Pubmed|24142252]
  • BKE06660 (Δ[gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTTACCCTCTCTTCA, downstream forward: _UP4_TAAGCAATAAGCCATCAAGC
  • BKK06660 (Δ[gene|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTTACCCTCTCTTCA, downstream forward: _UP4_TAAGCAATAAGCCATCAAGC

    • Labs working on this gene/protein

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

    • References

    Reviews

  • 27935846

    • Original publications

  • 12884008,9701821,11902719,11902719,22685134,22900538,22383849,24142252,22139509,26728461